Composite

Part:BBa_K1109005:Design

Designed by: Dr. zlem Darcansoy İşeri   Group: iGEM13_Baskent_Meds   (2013-09-23)


Anti-legionella unit


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 81
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 54
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Anti-legionella unit is a biobrick for suitable suffix and prefix regions. It was derived from synthetic oligonucleotides by tandem hybridization reactions, and amplified by PCR.


Source

Oligonucleotide sequence of the anti-legionella peptide was obtained from genomic sequence, and sequence of the PelB leader peptide was obtained from iGEM database.

References